Methods for inducing the differentiation of monocytes into functional dendritic cells
US6524855B2 · kind B2 · utility
Inventors
Key dates
| Filing date | Aug 13, 2001 |
| Grant date | Feb 25, 2003 |
| Priority date | — |
| Expiry date | Aug 13, 2021 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S977/924
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A method for inducing differentiation of monocytes contained in an extracorporeal quantity of a subject's blood into functional dendritic antigen presenting cells is provided. The monocytes are first treated by exposure to physical perturbation, irradiation in the presence of a photoactivatable agent capable of forming photoadducts with cellular DNA components, and/or treatment with a DNA binding agent. The treated monocytes are then incubated for a period of time sufficient to maximize the number of functional dendritic cells in the treated cell population. Functional dendritic cells generated from induced monocytes are incubated together with disease effector agents to enhance the presentation of at least one disease-causing antigen expressed by the disease effector agents.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.