Capillary electrophoretic method to detect target-binding ligands and to determine their relative affinities
US6524866B1 · kind B1 · utility
1Cited by
7References
28Claims
0Family size
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Key dates
| Filing date | Jun 21, 2000 |
| Grant date | Feb 25, 2003 |
| Priority date | — |
| Expiry date | Jun 21, 2020 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N27/44773
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The invention relates to capillary electrophoretic methods for detecting ligands or hit compounds that can bind to a selected target at or above a selected binding strength. The method allows one to rank various ligands based on their relative affinity, i.e., the relative stability of the target/ligand complex during capillary electrophoresis under selected conditions. The method also enables selective detection of strong-to-moderate binding hit compounds, even in the presence of high concentrations of weaker, competitive hit compounds.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.