Method for identifying components involved in signal transduction pathways in higher plants
US6525245B1 · kind B1 · utility
Assignee
Inventor
Key dates
| Filing date | May 22, 2000 |
| Grant date | Feb 25, 2003 |
| Priority date | — |
| Expiry date | May 22, 2020 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/8216
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to methods for determining nucleic acid sequences that encode components of signal transduction pathways in higher plants. The method comprises combining a portion of an AOX promoter linked in operable fashion to a reporter gene to detect nucleic acid sequences of components of the signal transduction pathways between mitochondria function and metabolic status and nuclear gene expression and the signal transduction pathways between branched chain amino acid biosynthetic pathways and nuclear gene expression. A polynucleotide that encodes a portion of an AOX promoter, AOX1a, operably linked to a luciferase reporter gene is provided. A recombinant vector, transformed cells, and transformed organisms containing this polynucleotide are disclosed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.