Method for enrichment of natural antisense messenger RNA
US6528262B1 · kind B1 · utility
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Key dates
| Filing date | Oct 6, 2000 |
| Grant date | Mar 4, 2003 |
| Priority date | — |
| Expiry date | Apr 13, 2021 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2330/10
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for enrichment of natural antisense mRNA which involves hybridization of cDNA obtained from sense RNA with cDNA obtained from antisense RNA, followed by DNA polymerase treatment of the sense-antisense hybrid DNA molecule. A natural antisense library can be generated by cloning of sense-antisense hybrid DNA molecules in a vector.
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