Targeted manipulation of genes in plants
US6528700B1 · kind B1 · utility
11Cited by
7References
32Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Nov 17, 1998 |
| Grant date | Mar 4, 2003 |
| Priority date | — |
| Expiry date | Nov 17, 2018 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2310/53
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods and compositions for altering a nucleotide sequence of interest in a plant is provided. The method involves introducing a chimeric oligonucleotide into a RNA residues with intervening DNA residues. The RNA blocks are homologous to a plant nucleotide sequence. The chimeric oligonucleotide is capable of folding to form a duplex oligonucleotide. The chimeric oligonucleotide is maintained within the nucleus of the plant whereby the alteration is introduced into the target sequences of the plant genome.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.