Optical detection in bio-separation device using a widened detection zone
US6529275B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jun 22, 2001 |
| Grant date | Mar 4, 2003 |
| Priority date | — |
| Expiry date | Jun 22, 2021 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N27/44721
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
In a bio-separation system, the detection zone for optical detection of sample analytes is located at a widened zone along the separation channel. In one embodiment of the present invention, the widened detection zone is a micro-bore collar having a micro-channel that coaxially surrounds the exit of a capillary column that defines a capillary channel. A separation support medium including a running buffer fills the capillary column and the collar. In another aspect of the present invention, incident radiation for detection of separated analytes is directed at the detection zone and/or emitted radiation is collected axially along the separation medium, instead of through the boundary walls of the detection zone. In a further aspect of the present invention, the optical detection configuration may be scaled up and implemented in a mult-channel CE system that comprises multiple capillary separation channels.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.