Cloning and copying on surfaces

US6534271B2 · kind B2 · utility

Assignee

• NOXXON Pharma AG · DE

Inventors

• Jens Furste · Berlin, DE
• Sven Klussmann · Berlin, DE
• Thomas Klein · Wehr, DE
• Günter Von Kiedrowski · Bochum, DE

Key dates

• Filing date: May 25, 2001
• Grant date: Mar 18, 2003
• Expiry date: May 25, 2021

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S977/902
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention provides for amplification methods for cloning and copying genetic material on surfaces as well as copying biological material insofar as, in a broader sense, it can be classified as a ligand-receptor system. The invention therefore relates in particular to a method for propagating ligands and receptors on at least two surfaces, comprising (a) immobilizing a first ligand on a first surface of a substantially solid phase; (b) adding a solution of receptors and binding complementary receptors to the first ligand; (c) transferring the receptor to a second surface and immobilizing the receptor at that location; (d) attaching an additional ligand to the immobilized receptor; and (e) transferring the additional ligand to the first surface and immobilizing it at that location, wherein the steps set forth above may be repeated,multiple times.