Tissue-engineered tubular construct having circumferentially oriented smooth muscle cells
US6537567B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Jul 2, 1998 |
| Grant date | Mar 25, 2003 |
| Priority date | — |
| Expiry date | Jul 2, 2018 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2533/40
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Improved methods for the production of tissue-engineered constructs, including muscular tissue constructs such as vascular constructs, are disclosed. The methods include the use of improved substrates for cell growth, improved cell culture media for cell growth, and the use of distensible bodies to impart pulsatile stretching force to lumens of constructs during growth. Also disclosed are improved products and methods for making those products, including substrates and cell culture media, for tissue engineering and tissue culture generally. Improved muscular tissue constructs, including vascular constructs, are also disclosed, which may be used in medicine for the repair or replacement of damaged natural structures. In an embodiment, a muscular, tubular tissue-engineered construct is prepared having a wall of mammalian smooth muscle cells oriented circumferentially about a lumen of the construct at a cell density of at least 107 cells/cc.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.