Prefunctionalized nucleotide and process for amplifying a sequence using a prefunctionalized nucleotide
US6537783B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Jan 11, 1999 |
| Grant date | Mar 25, 2003 |
| Priority date | — |
| Expiry date | Jan 11, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/686
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A target nucleic acid sequence amplification method wherein at least the target nucleic acid sequence, at least one oligonucleotide primer specific for the target sequence, one or more enzymatic activities and nucleotides are provided, and the target sequence is amplified under conditions suitable in particular for said enzymatic or activity or activities, is disclosed. According to the method, at least one of the nucleotides is a prefunctionalized nucleotide that differs from the other nucleotides at least in that it includes at least one unprotected reactive covalence function in at least one predetermined site of the base of said nucleotide, in order to give a prefunctionalized amplificati product including at least one such prefunctionalized nucleotide. Nucleotides for carrying out the method are also disclosed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.