Methods for the production of TcR&ggr;&dgr;+ T cells

US6537812B1 · kind B1 · utility

Assignee

• Hemosol, Inc. · CA

Inventors

• David Bell · Mississauga, CA
• Danna Lynn Skea · Mississauga, CA
• Phyllis Robin Hedge · Cambridge, CA

Key dates

• Filing date: Jul 30, 2001
• Grant date: Mar 25, 2003
• Expiry date: Jul 30, 2021

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2502/11
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The method for obtaining and expanding TcR&ggr;&dgr;+ T cells in culture is described. The method involves: 1) culturing cells from a sample containing TcR&ggr;&dgr;+ T cells or precursors thereof in a first culture medium comprising a T cell mitogen and at least two cytokines and 2) culturing the cells obtained in step 1) in a second culture medium comprising at least two cytolines. Preferably, the method comprises 1) culturing the cells in a first culture medium comprising (a) a T cell mitogen, (b) interleukin-2 and (c) interleukin-4, and 2) culturing the cells obtained in step 1) in a second culture medium comprising (i) interleukin-2 and (ii) interleukin-4 to obtain TcR&ggr;&dgr;+ T cells. The TcR&ggr;&dgr;+ T cells obtained by the method can be used in a variety of experimental, therapeutic and commercial applications.