Synthetic HIV-2 envelope genes containing modifications that lead to optimized expression in bacteria
US6538127B1 · kind B1 · utility
2Cited by
6References
7Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | May 18, 1995 |
| Grant date | Mar 25, 2003 |
| Priority date | — |
| Expiry date | May 18, 2015 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2740/16122
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides a method of synthesizing genes encoding unique HIV-1 and HIV-2 envelope proteins and their fragments, thereby allowing overexpression of these proteins in E. coli. The HIV envelope proteins and their fragments have been expressed at high levels as individual proteins or in fusion with other proteins. The HIV envelope proteins thus expressed in E. coli can be effectively used for the detection of exposure to HIV as well as the discrimination of HIV-1 and HIV-2.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.