Patent · US Expired

Method of removing nucleic acid contamination in amplification reactions

US6541204B2 · kind B2 · utility

8Cited by

1References

11Claims

0Family size

Assignee

Norwegian Institute of Fisheries & Aquaculture Ltd. · NO

Inventors

Inge Nilsen · Tromsø, NO
Erling Sandsdalen · Tromsø, NO
Even Stenberg · Tromsø, NO

Key dates

Filing date	Apr 10, 2000
Grant date	Apr 1, 2003
Priority date	—
Expiry date	Apr 10, 2020

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Q1/6848
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The present invention provides a method of removing nucleic acid contamination in an amplification reaction which comprises use of a thermolabile DNase and a method of preventing or reducing false positive results due to carry-over in a nucleic acid amplification reaction, said method comprising using a thermolabile DNase to degrade carried-over non-target double-stranded DNA present in the amplification reaction mixture. A thermolabile DNase from the shrimp Pandalus borealis has been identified which is suitable for use in the methods of the invention.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.