Preparation of labeled DNA
US6541227B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Oct 12, 1999 |
| Grant date | Apr 1, 2003 |
| Priority date | — |
| Expiry date | Oct 12, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/68
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A preparation method of labeled DNA and use thereof are disclosed. More specifically, the invention relates to a preparation method of labeled DNA by preparation of a complex triple-stranded DNA through an deoxyoligonucleotide complementary to the deoxyoligonucleotide sequence of the 3′ terminal sequence of a certain double-stranded DNA followed by replacement of the 3′ terminal region of the plus strand of said double-stranded DNA with at least one labeled dNTP, and also to said immobilization method of a DNA obtained from the preparation method of the labeled (or modified) DNAs onto a solid support. These subject matters are mainly useful for preparation of labeled DNA molecules.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.