Immortalized human stromal cell lines
US6541249B2 · kind B2 · utility
23Cited by
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9Claims
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Key dates
| Filing date | Dec 21, 2000 |
| Grant date | Apr 1, 2003 |
| Priority date | — |
| Expiry date | Jan 17, 2021 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12R2001/91
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for the culture of hematopoietic cells and/or production of human cytokines using immortalized human stromal cell lines is provided. These immortalized stromal cell lines condition media such that the ability of the hematopoietic stem cells to self-replicate and/or differentiate is maintained by the media or the ability of the committed progenitors to expand and/or differentiate is maintained by the media. Further provided are irradiated and non-irradiated immortalized human stromal cells that synthesize cytokines, such as, IL-7, IL-8, IL-11, FLT3L, SCF, LIF, M-CSF, TGF-beta 1, GM-CSF, MIP-1alpha, G-CSF, and MCP-1.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.