Nucleotide sequences encoding maize RAD51

US6541684B1 · kind B1 · utility

Assignees

• The Trustees of Columbia University in the City of New York · US
• PIONEER HI-BRED INTERNATIONAL, INC. · US

Inventors

• Benjamin A. Bowen · Berkeley, US
• Mark A. Chamberlin · Rue des Jonquilles, US
• Bruce Drummond · Des Moines, US
• John A. McElver · Durham, US
• Rodney Rothstein · Maplewood, US

Key dates

• Filing date: Feb 9, 1999
• Grant date: Apr 1, 2003
• Expiry date: Feb 9, 2019

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y02A40/146
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Nucleic acid sequences encoding two RAD51 recombinases active in maize plants are provided. cDNA sequences including the ZmRAD51 coding sequences and unique 3′-untranslated regions which are useful as RFLP probes, are also provided. The production of plasmids containing a nucleic acid sequence encoding a ZmRAD51 fusion protein, as well as the use of the plasmids to introduce the ZmRAD51 coding sequence into a host cell, such as maize cell, are also disclosed.