Method of checking the performance of a flow cytometer instrument and apparatus for executing said method as well as a standard kit therefore
US6542833B1 · kind B1 · utility
Assignee
Inventor
Key dates
| Filing date | Mar 10, 2000 |
| Grant date | Apr 1, 2003 |
| Priority date | — |
| Expiry date | Mar 10, 2020 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N15/1012
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for correcting the settings of a flow cytometer, designed for fast sample handling and counting, allowing about 500 samples per hour to be counted. The counting is based on the provision of data representing a PHA diagram (Pulse Height Analysis) of registered pulses, each indicating a passed cell or particle. To check the settings the user measures a standard sample of uniform microbeads 161 on the flow cytometer, and inserts information on a disk 162 in a computer arranged to process the measured data and to calculate: a plurality of particle counts on the same sample, a mean count, a standard deviation s and/or Coefficient of Variation CV, a signal mean value SM, a signal width (width of the bell-curve in the PHA-diagram). The parameters are compared to pre-set limits (165, 166, 167, 168) and the PHAS curve is compared to an ideal curve PHA0. A user help program for adjusting the flow cytometer is arranged to display typical symptoms on a computer screen, to indicate the possible defects and to recommend actions to remedy the problems, based on information in a library stored in the computer. Thereby a visit by a service engineer can often be avoided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.