Bioluminescent biosensor device
US6544729B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Jul 20, 2001 |
| Grant date | Apr 8, 2003 |
| Priority date | — |
| Expiry date | Jul 20, 2021 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N35/00069
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Disclosed are methods and devices for detection of bacteria based on recognition and infection of one or more selected strains of bacteria with bacteriophage genetically modified to cause production of an inducer molecule in the bacterium following phage infection. The inducer molecule is released from the infected bacterium and is detected by genetically modified bacterial bioreporter cells designed to emit bioluminescence upon stimulation by the inducer. Autoamplification of the bioluminescent signal permits detection of low levels of bacteria without sample enrichment. Also disclosed are methods of detection for select bacteria, and kits for detection of select bacteria based on the described technology.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.