Patent · US Expired

Multilayered microfluidic DNA analysis system and method

US6544734B1 · kind B1 · utility

236Cited by
114References
30Claims
0Family size

Inventors

Key dates

Filing dateDec 9, 1999
Grant dateApr 8, 2003
Priority date
Expiry dateDec 9, 2019

Classification

  • Technology area (CPC H)Electricity
  • CPC primaryH05K3/4629
  • WIPO fieldMeasurement
  • WIPO sectorInstruments

Abstract

A multilayered microfluidic DNA analysis system includes a cell lysis chamber, a DNA separation chamber, a DNA amplification chamber, and a DNA detection system. The multilayered microfluidic DNA analysis system is provided as a substantially monolithic structure formed from a plurality of green-sheet layers sintered together. The substantially monolithic structure has defined therein a means for heating the DNA amplification chamber and a means for cooling the DNA amplification chamber. The means for heating and means for cooling operate to cycle the temperature of the DNA amplification chamber as required for performing a DNA amplification process, such as PCR.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.