Rapid and sensitive proximity-based assay for the detection and quantification of DNA binding proteins
US6544746B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Aug 13, 2001 |
| Grant date | Apr 8, 2003 |
| Priority date | — |
| Expiry date | Aug 13, 2021 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/6875
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Methods to determine the activity of any and all DNA binding factors, proteins or fragments thereof based upon the detection of a change in a luminescence or fluorescence signal are provided. Preferably, a fluorescence donor is attached to a nucleic acid comprising one portion of a DNA binding element and a fluorescence acceptor is attached to a nucleic acid comprising the other portion of the same binding element. Alternatively, a microsphere bead is attached to a nucleic acid comprising one portion of a binding element and a luminescent moiety or fluorochrome is attached to a nucleic acid comprising the other portion of the same binding element. Binding of a DNA binding factor to the nucleic acid components affects a change in luminescence. These methods may also be used to detect mediating analytes, to diagnose diseases and/or screen for drugs that mediate the activity of DNA binding factors.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.