Preparation of erythropoietin by endogenous gene activation

US6544748B2 · kind B2 · utility

Assignee

• Roche Diagnostics GMBH · DE

Inventors

• Anne Stern · Penzberg, DE
• Michael Brandt · Iffeldorf, DE
• Konrad Honold · Penzberg, DE
• Johannes Auer · Schwaigen, DE
• Hans Koll · Oberroth, DE

Key dates

• Filing date: Nov 2, 2001
• Grant date: Apr 8, 2003
• Expiry date: Nov 2, 2021

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/75
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The invention relates to human cells which are capable, on the basis of an activation of the endogenous human EPO gene, of producing EPO in a sufficient amount and purity to make possible a cost-effective production of human EPO as a pharmaceutical preparation. The invention furthermore relates to a method for the preparation of such human EPO-producing cells, DNA constructs for the activation of the endogenous EPO in human cells, and a method for the large technical production of EPO in human cells.