Apparatus for glycosyltransferase-catalyzed saccharide synthesis
US6544778B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Dec 3, 2001 |
| Grant date | Apr 8, 2003 |
| Priority date | — |
| Expiry date | Dec 3, 2021 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/819
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for preparing saccharide compositions is disclosed. The method is reiterative and comprises the following three steps.(i) A glycosyltransferase capable of transferring a preselected saccharide unit to an acceptor moiety is isolated by contacting the acceptor moiety with a mixture suspected of containing the glycosyltransferase under conditions effective to bind the acceptor moiety and the glycosyltransferase and thereby isolate the glycosyltransferase. The acceptor moiety is a protein, a glycoprotein, a lipid, a glycolipid, or a carbohydrate.(ii) The isolated glycosyltransferase is then used to catalyze the bond between the acceptor moiety and the preselected saccharide unit.(iii) Steps (i) and (ii) are repeated a plurality of times with the intermediate product obtained in the first iteration of the method being used as the acceptor moiety of the second iteration.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.