Multiparametric fluorescence in situ hybridization

US6548259B2 · kind B2 · utility

Assignee

• Yale University · US

Inventors

• David C. Ward · Guilford, US
• Michael Speicher · Ottobrunn, DE
• Stephen Gwyn Ballard · Hamden, US
• John T. Wilson · Augusta, US

Key dates

• Filing date: Nov 20, 2001
• Grant date: Apr 15, 2003
• Expiry date: Nov 20, 2021

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6841
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention relates to a set of combinatorially labeled oligonucleotide probes each member thereof: (i) having a predetermined label distinguishable from the label of any other member of said set, and (ii) being capable of specifically hybridizing with a predetermined chromosome or nucleic acid molecule, and to the use of such molecules, alone or in concert with nucleic acid amplification methods.