Methods for identifying human cell lines useful for endogenous gene activation, isolated human lines identified thereby, and uses thereof

US6548296B1 · kind B1 · utility

Assignee

• Roche Diagnostics GMBH · DE

Inventors

• Anne Stern · Penzberg, DE
• Michael Brandt · Iffeldorf, DE
• Konrad Honold · Penzberg, DE
• Johannes Auer · Schwaigen, DE
• Hans Koll · Oberroth, DE
• Reinhard Franze · Penzberg, DE
• Ulrich Pessara · Weilheim, DE

Key dates

• Filing date: Jul 10, 1998
• Grant date: Apr 15, 2003
• Expiry date: Jul 10, 2018

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/75
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The invention concerns human cells which, due to an activation of the endogenous human EPO gene, are able to produce EPO in an adequate quantity and purity to enable a cost-effective production of human EPO as a pharmaceutical preparation. Furthermore the invention concerns a process for the production of such human EPO-producing cells, DNA constructs for the activation of the endogenous EPO gene in human cells as well as a process for the large-scale production of EPO in human cells.