Production of heterologous protein in milk of transgenic non-human mammals comprising a construct with an intron
US6548735B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Jan 9, 1997 |
| Grant date | Apr 15, 2003 |
| Priority date | — |
| Expiry date | Jan 9, 2017 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2830/85
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Proteinaceous products can be produced by transgenic animals having genetic constructs integrated into their genome. The construct comprises a 5′-flanking sequence from a mammalian milk protein gene (such as beta-lactoglobulin) and DNA coding for a heterologous protein other than the milk protein (for example a serin protease such as alpha1-antitrypsin or a blood factor such as Factor VIII or IX). The protein-coding DNA comprises at least one, but not all, of the introns naturally occurring in a gene coding for the heterologous protein. The 5′-flanking sequence is sufficient to drive expression of the heterologous protein.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.