Methods for creating transgenic animals
US6548740B1 · kind B1 · utility
1Cited by
5References
11Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Feb 11, 2000 |
| Grant date | Apr 15, 2003 |
| Priority date | — |
| Expiry date | Feb 11, 2020 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2740/13051
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides improved methods and compositions for the generation of transgenic non-human animals. The present invention permits the introduction of exogenous nucleic acid sequences into the genome of unfertilized eggs (e.g., pre-maturation oocytes and pre-fertilization oocytes) by microinjection of infectious retrovirus into the perivitelline space of the egg. The methods of the present invention provide an increased efficiency of production of transgenic animals with a reduced rate of generating animals which are mosaic for the presence of the transgene.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.