Developmental competence for assisted reproduction and nuclear transfer in pigs
US6548741B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Apr 9, 2001 |
| Grant date | Apr 15, 2003 |
| Priority date | — |
| Expiry date | Apr 9, 2021 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2517/10
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
An optimum time has been discovered for obtaining porcine oocytes with improved developmental competence. Harvested oocytes are matured by culturing in vitro, and then activated ˜42-46 hours after beginning of the culture period. Alternatively, ovulation is induced in a donor female using a gonadotrophic hormone (optionally monitored by ultrasonography), maturation is allowed to proceed in vivo, and then the harvested oocyte is activated ˜44-48 hours after inducing ovulation. Outside the optimal time frame, the ability of the oocytes to undergo parthenogenetic activation declines markedly. These methods overcome problems encountered with previous protocols for nuclear transfer and assisted reproduction in the pig.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.