Expression system for producing proteins
US6551797B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Feb 28, 2001 |
| Grant date | Apr 22, 2003 |
| Priority date | — |
| Expiry date | Feb 28, 2021 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/80
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
An expression system for producing a protein in a filamentous fungus, consisting of a) a host organism selected from the species Trametes and Polyporus and b) a DNS vector containing a selection marker gene. This selection marker gene code is a protein which allows the selection of positive transformants after the transformation of the host organism and which is selected from the group of: antibiotics-resistant genes coding for proteins which eliminate the growth-inhibitory effect of antibiotics against which the host organism is not resistant. Genes coding proteins which are capable of a color-causing reaction; and genes complementing a genetic defect of the host organism (auxotrophy), the expression of the selection marker gene is controlled by at least one active genetic regulation element in the host organism, and c) a DNS vector containing a gene which codes the protein to be produced. The expression of this gene and optionally, the secretion of the protein so produced are controlled by an active genetic regulation element in the host organism. The DNS vector containing a section marker gene and the DNS vector containing the gene which codes the protein to be produced can also…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.