Process for preparing purified dimer of bone-derived factor
US6551801B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Jul 7, 1999 |
| Grant date | Apr 22, 2003 |
| Priority date | — |
| Expiry date | Jul 7, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K14/51
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A process for preparing a purified dimer of a bone-derived factor, which comprises subjecting an inclusion body of a bone-derived factor produced by genetic engineering to the following steps a) to e) in sequence:a) the step of treating an inclusion body of a bone-derived factor with a unfolding agent to prepare a solubilized monomer;b) the step of treating the solubilized monomer with a refolding solution to prepare a dimer;c) the step of subjecting the refolded dimer to ultrafiltration and solvent replacement;d) the step of subjecting the dimer solution prepared above to isoelectric precipitation; ande) the step of subjecting the isoelectrically precipitated dimer to reverse-phase chromatography.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.