Methods for producing and transforming cassave protoplasts
US6551827B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Feb 1, 1999 |
| Grant date | Apr 22, 2003 |
| Priority date | — |
| Expiry date | Feb 1, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/8201
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention relates to a method for producing protoplasts of cassava or closely related species, which protoplasts are capable of regeneration into plants. The method comprises producing friable embryogenic callus from explants of cassava or closely related species and isolating protoplasts from said friable embryogenic callus. The invention also concerns protoplasts obtainable by said method. The invention further relates to a method for transforming such a protoplast of cassava or closely related species, and transformed protoplasts obtainable thereby. In addition, the invention concerns a method for regenerating plants from these protoplasts and a cassava plant or closely related species obtainable thereby.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.