Method for photolytically deprotecting immobilized nucleoside derivatives, especially in the production of DNA chips

US6552182B2 · kind B2 · utility

Assignee

• NIGU Chemie GmbH · DE

Inventors

• Klaus-Peter Stengele · Pleiskirchen, DE
• Heinrich Giegrich · Waldkraiburg, DE

Key dates

• Filing date: Sep 10, 2001
• Grant date: Apr 22, 2003
• Expiry date: Sep 10, 2021

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y02P20/55
• WIPO field: Organic fine chemistry
• WIPO sector: Chemistry

Abstract

The invention relates to a method for the specific photolytic deprotection of nucleoside derivatives that are immobilized on a substrate, especially for use in the production of DNA chips. Said method is characterized in that a gel or viscous liquid layer is applied on the nucleoside derivatives that are immobilized on a substrate. Said gel or viscous liquid contains one or more polymer compounds and at least one representative from the group comprising water, water/C1-C4 alcohol mixtures and polar aprotic solvents. For initiating the deprotection, the nucleoside derivates are irradiated. This method favors a rapid, clean and complete removal of the photolabile protective groups from the nucleoside derivatives, which results in the required purity of the synthesized nucleotide or oligonucleotide sequences.