System for detection of a functional interaction between a compound and a cellular signal transduction component
US6555325B1 · kind B1 · utility
Assignee
Inventor
Key dates
| Filing date | Jun 12, 2000 |
| Grant date | Apr 29, 2003 |
| Priority date | — |
| Expiry date | Jun 12, 2020 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/4719
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention makes available a rapid, reproducible, robust assay system for screening and identifying pharmaceutically effective compounds that specifically interact with and modulate the activity of a cellular protein, e.g., a receptor or ion channel. The subject assay enables rapid screening of large numbers of compounds to identify those which act as an agonist or antagonist to the bioactivity of the cellular protein. In this system, the first cell is treated with a compound, and functional interaction of this compound with a cellular receptor yields a secreted signal. A second cell, bearing a receptor for this secreted signal, makes use of an indicator gene in a signaling pathway coupled to this second receptor. The subject assays include methods of identifying compounds which specifically modulate, for example, heterologous receptors coupled to the pheromone response pathway in yeast. The subject assays are particularly amenable to the identification of specific agonists and antagonists of G protein-coupled receptors.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.