Fluorescent detection method for microorganisms based on nitrocoumarins
US6555332B2 · kind B2 · utility
3Cited by
4References
9Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | May 4, 2001 |
| Grant date | Apr 29, 2003 |
| Priority date | — |
| Expiry date | May 4, 2021 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/26
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A nitrocoumarin compound or one of its derivatives which are fluorescent in the reduced state, and the use of these compounds for detecting the presence or absence of at least one microorganism. The invention concerns the use of these compounds, methods for isolating and detecting microorganisms or a group of microorganisms in a sample likely to contain them, and various applications thereof. Also disclosed is a method for detecting nitroaryl reductase activity in a bacteria culture medium.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.