Process for production of mammalian cell lines
US6555377B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Apr 27, 2000 |
| Grant date | Apr 29, 2003 |
| Priority date | — |
| Expiry date | Apr 27, 2020 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2510/04
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
Described is a process for generating a mammalian cell line from primary mammalian cells, comprising the step of:a) pre-treating a culture of said primary mammalian cells or a suspension thereof with at least one glucocorticoid,b) optional step comprising obtaining a suspension of said pre-treated culture of step a),c) transferring into the pre-treated cells of the suspension of step a) or b) at least one nucleic acid vector which is not of retroviral origin and which is competent to immortalize said pre-treated cells andd) culturing the transferred cells of step c).Furthermore, mammalian cell lines and cells obtainable by the described process are provided as well as pharmaceutical and diagnostic compositions containing such cells.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.