UDP-galactose: &bgr;-N-acetyl-glucosamine &bgr;-1,4-galactosyl-transferase, &bgr;4Gal-T2
US6558934B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Jul 17, 1998 |
| Grant date | May 6, 2003 |
| Priority date | — |
| Expiry date | Jul 17, 2018 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N9/1051
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A novel gene defining a novel enzyme in the UDP-D-galactose: &bgr;-N-acetyl-glucosamine &bgr;-1,4-galactosyltransferase family, termed &bgr;4Gal-T2, with unique enzymatic properties is disclosed. The enzymatic activity of &bgr;4Gal-T2 is shown to be distinct from that of previously identified enzymes of this gene family. The invention discloses isolated DNA molecules and DNA constructs encoding &bgr;4Gal-T2 and derivatives thereof by way of amino acid deletion, substitution or insertion exhibiting &bgr;4Gal-T2 activity, as well as cloning and expression vectors including such DNA, cells transfected with the vectors, and recombinant methods for providing &bgr;4Gal-T2. The enzyme &bgr;4Gal-T2 and &bgr;4Gal-T2-active derivatives thereof are disclosed, in particular soluble derivatives comprising the catalytically active domain of &bgr;4Gal-T2. Further, the invention discloses methods of obtaining &bgr;-1,4-galactosyl glycosylated saccharides, glycopeptides or glycoproteins by use of an enzymically active &bgr;4Gal-T2 protein or fusion protein thereof or by using cells stably transfected with a vector including DNA encoding an enzymatically active &bgr;4Gal-T2 protein as an expression …
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.