Permanent amniocytic cell line, its production and use for the production of gene transfer vectors

US6558948B1 · kind B1 · utility

Inventors

• Stefan Kochanek · Ulm, DE
• Gudrun Schiedner · Cologne, DE

Key dates

• Filing date: Nov 16, 2000
• Grant date: May 6, 2003
• Expiry date: Nov 16, 2020

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2710/10352
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention relates to a permanent amniocytic cell line comprising at least one nucleic acid which brings about expression of the gene products of the adenovirus E1A and E1B regions. The present invention further relates to the production of a permanent amniocytic cell line and to its use for producing gene transfer vectors and/or adenovirus mutants. Further aspects are the use of amniocytes and of the adenoviral gene products of the E1A and E1B regions for producing permanent amniocytic cell lines.