Recombinant allergen, fragments thereof, corresponding recombinant DNA molecules, vectors and hosts containing the DNA molecules, diagnostic and therapeutic uses of said allergens and fragments

US6559120B2 · kind B2 · utility

Assignee

• Pharmacia AB · SE

Inventors

• Tanja Ball · Gleisdorf, AT
• Susanne Vrtala · Wien, AT
• Wolfgang Sperr · Wien, AT
• Peter Valent · Wien, AT
• Markus Susani · Salzburg, AT
• Dietrich Kraft · Wien, AT
• Rudolf Valenta · Theresienfeld, AT
• Sylvia Laffer · Wien, AT

Key dates

• Filing date: Mar 20, 2001
• Grant date: May 6, 2003
• Expiry date: Mar 20, 2021

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/00
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

A recombinant DNA molecule comprising a nucleotide sequence (I) which codes for a polypeptide displaying the antigenicity of one, two or more of the Phi p I epitope clones (28, 34, 41, 42, 43, 45, 50, 52, 64, 80, 85, 86, 95, 97, 98, 103, 108, 109, 113, 114), with the amino acid sequences defined in SEQ ID NOS: 5, 7, 9 and 12-28, and preferably being derived from grasses or monocotyledonic plants, or a nucleotide sequence (II) which hybridizes with such a nucleotide sequence (I) under conditions of high stringency. Polypeptides displaying the antigenicity of one, two or more of the Phi p I epitope clones (28, 34, 41, 42, 43, 50, 52, 64, 80, 85, 86, 95, 97, 98, 103, 108, 109, 113, 114). Recombinant expression vectors containing the recombinant molecule and host cells transformed with the vector. Diagnostic methods based on utilizing the polypeptides in immunoassays for humoral antibodies and cellular reactions.