Precipitation of proteins from organic solutions
US6562952B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Oct 31, 2000 |
| Grant date | May 13, 2003 |
| Priority date | — |
| Expiry date | Oct 31, 2020 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA61K9/1688
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A process for forming small micron-sized (1-10 &mgr;m) protein particles is provided wherein a protein, a solvent system for the protein and an antisolvent for the protein solvent system are contacted under conditions to at least partially dissolve the protein solvent system in the antisolvent, thereby causing precipitation of the protein. The solvent system is made up of at least in part of a halogenated organic alcohol, most preferably 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP). Preferably, a solution of the protein in the solvent system is sprayed through a nozzle into a precipitation zone containing the antisolvent (preferably CO2) under near- or supercritical conditions.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.