In vivo electroporation method for early stage embryo of chickens
US6563017B2 · kind B2 · utility
10Cited by
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2Claims
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Assignee
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Key dates
| Filing date | Sep 13, 1999 |
| Grant date | May 13, 2003 |
| Priority date | — |
| Expiry date | Sep 13, 2019 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA01K2267/01
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A gene is transferred to the early stage chicken embryo by electroporation with placing the electrode tips to hold both ends of the embryo. Transfer of the desired gene to the early stage embryo was detected using the &bgr;-galactosidase gene of E coli as a reporter gene. The expression of the gene in the early stage embryo of chicken was thus confirmed. This method for transferring a gene into undifferentiated cells is simple to implement, highly efficient in transferring a gene, and applicable to various animal species.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.