Gene sequencer and method for determining the nucleotide sequence of a chromosome
US6566069B2 · kind B2 · utility
11Cited by
4References
18Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | May 21, 2001 |
| Grant date | May 20, 2003 |
| Priority date | — |
| Expiry date | May 21, 2021 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N35/00069
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for determining the nucleotide sequence of a chromosome. The chromosome is hydrolyzed to form a mixture of oligonucleotides having unknown nucleotide sequences. The mixture is exposed to a library of oligonucleotide probe pairs having known sequences. The probe pairs are located so that they hybridize to outer opposed sections of the chromosomal oligonucleotides. The use of oligonucleotide probe pairs provides for sequencing of relatively long unknown DNA sequences using relatively short oligonucleotide probes.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.