Immortalized cell line derived from normal human skin tissues
US6566136B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Sep 18, 2000 |
| Grant date | May 20, 2003 |
| Priority date | — |
| Expiry date | Sep 18, 2020 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2510/04
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A human keratinocyte cell line immortalized by at least one functional tumor gene of DNA viral origin characterized in that it is: (1) non-tumorigenic, (2) conserves the capacity for differentiation and for the expression of proteins and of enzymes expressed by normal differentiated keratinocytes even after an elevated number of passages in culture; and (3) forms a stratified and polarized epithelium having a stratum corneum ortho-keratinocyte, if cultivated in an organo-typical culture in a medium without serum and without a layer of nourishing cells. An improved process to immortalize human skin cells to obtain immortalized keratinocytes. Also, the use of keratinocytes for immunological, pharmacological, photo- and chemical-toxicological analyzes of skin reaction and for expression of heterologous genes and for the construction of artificial skin.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.