Compositions and methods for modifying toxic effects of proteinaceous compounds

US6566500B1 · kind B1 · utility

Assignee

• The Board of Regents of the University of Texas System · US

Inventors

• Ellen S. Vitetta · Dallas, US
• Victor F. Ghetie · Dallas, US
• Joan E. Smallshaw · Dallas, US
• Roxana G. Baluna · Richardson, US

Key dates

• Filing date: Mar 30, 2000
• Grant date: May 20, 2003
• Expiry date: Mar 30, 2020

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/30
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The present invention provides methods to produce immunotoxins (ITs) and cytokines with a reduced ability to promote vascular leak syndrome (VLS). The invention also provides ITs and cytokines which have been mutated to lack amino acid sequences which induce VLS. Also disclosed are methods for producing peptides that inhibit the induction of VLS by ITs and cytokines. Also disclosed are peptides comprising the (x)D(y) sequence to promote the extravasation of other molecules. Toxins mutated in the (x)D(y) motif or active site residues are disclosed for used in vaccines.