Mutated 5-enolpyruvylshikimate-3-phosphate synthase, gene coding for said protein and transformed plants containing said gene
US6566587B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Jan 20, 1998 |
| Grant date | May 20, 2003 |
| Priority date | — |
| Expiry date | Jan 20, 2018 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/8275
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to DNA molecules of plant origin encoding a modified 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) enzyme wherein the a first EPSPS coding sequence that normally encodes a threonine residue of a mature EPSPS sequence is modified to encode isoleucine of the mature EPSPS sequence, and a second EPSPS coding sequence that normally encodes a proline residue of a mature EPSPS sequence is modified to encode serine of the mature EPSPS sequence, wherein the first and second residues are respectively located at relatively positions 102 and 106 of a mature EPSPS sequence encoded by said DNA molecule; and the production of a transgenic plant resistant or tolerant to a herbicide of the phosphonomethylglycine family, e.g., glyphosate. The mutated enzyme, which substantially maintains the catalytic activity of the wild-type enzyme, allows for increased tolerance or tolerance of the plant to a herbicide of the phosphonomethylglycine family, and allows for the substantially normal growth or development of the plant, its organs, tissues or cells.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.