Method and apparatus for performing biological reactions on a substrate surface
US6569674B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Jan 26, 2000 |
| Grant date | May 27, 2003 |
| Priority date | — |
| Expiry date | Jan 26, 2020 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2035/00267
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention provides a method and an improved apparatus for removing gas bubbles from a reaction chamber comprising a flexible layer removably affixed to a substrate layer having a multiplicity of oligonucleotide binding sites disposed thereon, in which biological reactions are performed. The invention specifically relates to methods and apparatus for removing gas bubbles from a reaction chamber wherein target molecules contained in a sample fluid are reacted with probe molecules immobilized on a substrate having an array of oligonucleotide binding sites. The arrays are covered with a flexible, gas permeable layer that permits mixing of the sample fluid on the biochip and removal of gas bubbles from the fluid by use of a means for facilitating diffusion of gas bubbles across the flexible, gas permeable layer.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.