Determination of recombinant glycosylated proteins and peptides in biological fluids
US6573055B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Apr 21, 1998 |
| Grant date | Jun 3, 2003 |
| Priority date | — |
| Expiry date | Apr 21, 2018 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K16/22
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Polyclonal antibodies can be produced that reacts with recombinant EPO and its degradation products but not with native EPO. This antibody precipitation can be used to identify those glycopeptides that are uniquely reactive. These glycopeptides can be produced on preparative scale and used in the production of monoclonal antibodies which are screened against the original EPO and glycopeptides to select antibodies reactive to the specific glycopeptides an recombinant EPO but not to native human EPO. The monoclonal antibodies so selected are incorporated in a conventional ELISA and used to monitor urine and other bodily samples taken from athletes, either human or animal, and patients for presence and level of recombinant peptides or proteins. Alternatively, the polyclonal antibody can be used directly to produce ELISA tests.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.