Method of sample introduction for chromatographic systems
US6576125B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Aug 17, 2001 |
| Grant date | Jun 10, 2003 |
| Priority date | — |
| Expiry date | Oct 20, 2021 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2030/347
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An improved method of introducing sample into a low-pressure flow stream of a high-pressure chromatography system to improve efficiency and significantly shorten the time of the separation process through a chromatography column is described. The method comprises injecting samples in a modifier liquid flow stream upstream of a mixing column that combines two independent flow streams of a liquid modifier with a highly compressed solvating fluid. Focusing can be controlled at the column head by changing the ratios of the two flow streams. Total flow may also be adjusted to speed the application of the sample onto the column during injection. The method improves the timing and efficiency of a supercritical fluid chromatography system and can reduce the amount of modifier added to the mobile phase flow stream.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.