Assay procedure using fluorogenic tracers
US6576419B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Jul 10, 1997 |
| Grant date | Jun 10, 2003 |
| Priority date | — |
| Expiry date | Jul 10, 2017 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6818
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Fluorescent energy transfer dyes capable of moving between a more stacked configuration to exhibit fluorescent quenching and a more spaced configuration to exhibit fluorescence can be conjugated to a peptide epitope or nucleic acid for use in the detection of an unknown antibody in bulk solution. The resulting labeled peptide reagent can be used in an immunoassay procedure by placing it in bulk solution along with the unknown antibody to be detected. When the antibody binds to the peptide epitope, the pair of dyes carried by the peptide epitope will have their configuration altered from a stacked to an unstacked configuration and will exhibit a fluorescent increase in response thereto.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.