Methods for implanting cells

US6579313B2 · kind B2 · utility

Assignee

• The Bringham and Women's Hospital, Inc. · US

Inventors

• Victor J. Dzau · Newton, US
• Richard E. Pratt · Newton, US
• Michael J. Mann · San Francisco, US
• Afshin Ehsan · Boston, US
• Daniel P. Griese · Boston, US

Key dates

• Filing date: Jan 18, 2002
• Grant date: Jun 17, 2003
• Expiry date: Jan 18, 2022

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S623/926
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

A method for implanting cells onto a prosthesis includes the steps of: (a) providing a prosthesis including a porous tube, where at least 25% of the pores on the inner surface of the tube have diameters of more than about 40 &mgr;m, at least 25% of the pores on the outer surface of the tube have diameters of less than about 30 &mgr;m, and the tube includes a substantially continuous layer of a biocompatible material; (b) contacting the prosthesis with a suspension of cells; and (c) providing a pressure differential between the inner surface and the outer surface, whereby the cells are retained in the pores of the inner surface. A method for obtaining an endothelial cell culture from a blood sample involved:(a) obtaining a sample for mononuclear cells from a blood sample; and (b) culturing the sample of mononuclear cells, without further cell separation, on a cell adhesive polymer-coated solid support in the presence of endothelial growth factors.