Patent · US Expired

Modulation of &ggr;-secretase activity

US6579689B2 · kind B2 · utility

1Cited by

0References

6Claims

0Family size

Assignee

SCIOS INC. · US

Inventors

Barbara Cordell · Palo Alto, US
Jeffrey N. Higaki · Mountain View, US
Mitchell Mutz · San Diego, US

Key dates

Filing date	May 9, 2001
Grant date	Jun 17, 2003
Priority date	—
Expiry date	May 9, 2021

Classification

Technology area (CPC A)Human Necessities
CPC primaryA61K38/00
WIPO fieldPharmaceuticals
WIPO sectorChemistry

Abstract

The present invention provides cell-free &ggr;-secretase activity. The method of the invention utilizes a membrane source of APP/&ggr;-secretase mixture in the assay to determine factors that may enhance or decrease enzymatic activity affecting &bgr;-amyloid peptide production. The cell membranes used in the assay may be from cells expressing an endogenous APP or, preferably, cells expressing a recombinant APP. The APP may be full-length or a fragment capable of being proteolytically cleaved by &ggr;-secretase. In addition, the APP expressed in the cells may have one or more mutation, such as a point mutation, small deletion, etc.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.