Phosphopantetheinyl transferases and uses thereof

US6579695B1 · kind B1 · utility

Assignees

• President and Fellows of Harvard College · US
• The Regents of the University of California · US

Inventors

• Ralph H. Lambalot · Wrentham, US
• Amy M. Gehring · Beulah, US
• Ralph Reid · San Rafael, US
• Christopher Walsh · Wellesley, US

Key dates

• Filing date: Oct 11, 1996
• Grant date: Jun 17, 2003
• Expiry date: Oct 11, 2016

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/569
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The invention pertains to isolated phosphopantetheinyl transferases, such as the E. coli acyl carrier protein synthase, which transfer a phosphopantetheinyl group onto a substrate. The enzyme can be purified from a natural source, produced recombinantly, or synthetically. Accordingly, the invention provides compositions and kits including phosphopantetheinyl transferases and host cells expressing phosphopantetheinyl transferases. The invention also provides nucleic acids encoding phosphopantetheinyl transferases and vectors comprising such nucleic acids. The invention further provides methods for phosphopantetheinylating a substrate in vitro or in vivo and methods for producing antibiotics in vitro or in vivo.