Carbazolylvinyl dye protein stains
US6579718B1 · kind B1 · utility
32Cited by
1References
34Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Aug 4, 2000 |
| Grant date | Jun 17, 2003 |
| Priority date | — |
| Expiry date | May 4, 2021 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/25375
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention describes a variety of substituted and unsubstituted carbazolylvinyl dyes and their use for detecting and quantifying poly(amino acids), including peptides, polypeptides and proteins. The labeled proteins or peptides are highly colored, but are also detected by their strong fluorescence enhancement. Poly(amino acids) are detected in solution, in electrophoretic gels, and on solid supports, including blots and dipsticks. The present method of staining is highly sensitive, extremely facile, and relatively non-selective and can be accomplished without the use of organic solvent additives.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.